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FILM acquisition of an anti-tubulin-APC antibody applied to Jurkat T cells (unlabeled cells) and <t>Raji</t> B cells (blue arrowheads) co-cultured on glass and then PFA-fixed and permeabilized. Blue arrowhead labeling is based on an image (not shown) of CMAC staining for pre-labeling of the Raji B cells before setting up the co-culture. Boxed regions are further analyzed in the respective figures or figure panels specified by the labels.
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FILM acquisition of an anti-tubulin-APC antibody applied to Jurkat T cells (unlabeled cells) and <t>Raji</t> B cells (blue arrowheads) co-cultured on glass and then PFA-fixed and permeabilized. Blue arrowhead labeling is based on an image (not shown) of CMAC staining for pre-labeling of the Raji B cells before setting up the co-culture. Boxed regions are further analyzed in the respective figures or figure panels specified by the labels.
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FILM acquisition of an anti-tubulin-APC antibody applied to Jurkat T cells (unlabeled cells) and Raji B cells (blue arrowheads) co-cultured on glass and then PFA-fixed and permeabilized. Blue arrowhead labeling is based on an image (not shown) of CMAC staining for pre-labeling of the Raji B cells before setting up the co-culture. Boxed regions are further analyzed in the respective figures or figure panels specified by the labels.

Journal: bioRxiv

Article Title: Ångström Resolution with Flow Immunofluorescence Localization Microscopy (FILM)

doi: 10.64898/2025.12.16.694546

Figure Lengend Snippet: FILM acquisition of an anti-tubulin-APC antibody applied to Jurkat T cells (unlabeled cells) and Raji B cells (blue arrowheads) co-cultured on glass and then PFA-fixed and permeabilized. Blue arrowhead labeling is based on an image (not shown) of CMAC staining for pre-labeling of the Raji B cells before setting up the co-culture. Boxed regions are further analyzed in the respective figures or figure panels specified by the labels.

Article Snippet: As a model for the immunological synapse, human B cell lymphoma Raji cells (American Type Culture Collection, #CCL-86) and Jurkat cells (clone E6-1, American Type Culture Collection, #TIB-152) were cultured in RPMI-1640 medium (Biowest, #L0501) supplemented with 10% (vol/vol) fetal bovine serum (Eximus Group, #BS-2022-500) and 2 mM L-glutamine (Thermo Fisher Scientific, #11539876).

Techniques: Cell Culture, Labeling, Staining, Co-Culture Assay